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Obovatol Induces Apoptosis in Non‐small Cell Lung Cancer Cells via C/EBP Homologous Protein Activation

Kim, Heejeong, Shin, Eun Ah, Kim, Chang Geun, Lee, Dae Young, Kim, Bonglee, Baek, Nam‐In, Kim, Sung‐Hoon
Phytotherapy research 2016 v.30 no.11 pp. 1841-1847
Magnolia obovata, antioxidants, apoptosis, bark, caspases, cyclins, cytotoxicity, endoplasmic reticulum stress, lung neoplasms, neoplasm cells
Although obovatol, a phenolic compound from the bark of Magnolia obovata, was known to have antioxidant, neuroprotective, antiinflammatory, antithrombotic and antitumour effects, its underlying antitumour mechanism is poorly understood so far. Thus, in the present study, the antitumour molecular mechanism of obovatol was investigated in non‐small cell lung cancer cells (NSCLCs). Obovatol exerted cytotoxicity in A549 and H460 NSCLCs, but not in BEAS‐2B cells. Also, obovatol increased sub‐G1 accumulation and early and late apoptotic portion in A549 and H460 NSCLCs. Consistently, obovatol cleaved PARP, activated caspase 9/3 and Bax and attenuated the expression of cyclin D1 in A549 and H460 NSCLCs. Interestingly, obovatol upregulated the expression of endoplasmic reticulum stress proteins such as C/EBP homologous protein (CHOP), IRE1α, ATF4 and p‐elF2 in A549 and H460 NSCLCs. Conversely, depletion of CHOP blocked the apoptotic activity of obovatol to increase sub‐G1 accumulation in A549 and H460 NSCLCs. Overall, our findings support scientific evidences that obovatol induces apoptosis via CHOP activation in A549 and H460 NSCLCs. Copyright © 2016 John Wiley & Sons, Ltd.