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Mechanism of the combined anti-bacterial effect of green tea extract and NaCl against Staphylococcus aureus and Escherichia coli O157:H7

Author:
Nakayama, Motokazu, Shigemune, Naofumi, Tsugukuni, Takashi, Jun, Hitomi, Matsushita, Tomoyo, Mekada, Yoko, Kurahachi, Masahiro, Miyamoto, Takahisa
Source:
Food control 2012 v.25 no.1 pp. 225-232
ISSN:
0956-7135
Subject:
Escherichia coli O157, Staphylococcus aureus, bacteria, catechin, electron microscopes, enzyme activity, enzyme substrates, green tea, polyacrylamide gel electrophoresis, secretion, sodium chloride, surface proteins, survival rate
Abstract:
The mechanism of the combined anti-bacterial effect of green tea extract (GTE) and NaCl against Staphylococcus aureus NBRC 13276 and Escherichia coli O157:H7 was investigated. After treatment for 1 h, GTE was more effective against S. aureus than E. coli O157:H7, and combined GTE/NaCl treatment caused greater cellular damage in S. aureus NBRC 13276, where it was bactericidal, than E. coli O157:H7. Compared to treatment with 1.0 mg/mL GTE, which had no effect on the survival rate of E. coli O157:H7 after 48 h, treatment with 4% NaCl alone caused greater cellular damage. Moreover, bacteria pretreated with NaCl showed delayed growth in the presence of GTE. It is therefore likely that susceptibility of E. coli O157:H7 to GTE was increased by exposure to NaCl. E. coli O157:H7 pretreated with GTE and NaCl did not multiply in the presence of GTE. Visualization of the catechin components of GTE-treated bacteria using an electron microscope and SDS-PAGE analysis of cell proteins showed that GTE attached to proteins on the surface of the bacteria to form high-molecular weight complexes, suggesting the possibility that GTE inhibits the uptake and secretion of substrates and inhibits enzyme activity. Notably, after the GTE treatment for 1 h, both bacterial strains suffered injury but recovered by cultivation in rich medium. The damage and aggregation of proteins caused by GTE treatment were repaired upon treatment with LP diluent.
Agid:
572199