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Transcriptional profiles in bursal B-lymphoid DT40 cells infected with very virulent infectious bursal disease virus

Author:
Quan, Rong, Zhu, Shanshan, Wei, Li, Wang, Jing, Yan, Xu, Li, Zixuan, Liu, Jue
Source:
Virology journal 2017 v.14 no.1 pp. 7
ISSN:
1743-422X
Subject:
cell death, immunosuppression, signal transduction, genes, Infectious bursal disease virus, mitogen-activated protein kinase, bursa of Fabricius, cytokines, viruses, pathogenesis, gene expression regulation, virulence, B-lymphocytes, Toll-like receptors, avian leukosis, messenger RNA, models, DNA microarrays, transcription (genetics), chickens, antigen presentation, inflammation, reverse transcriptase polymerase chain reaction
Abstract:
BACKGROUND: Infectious bursal disease virus (IBDV) causes a highly contagious, immunosuppressive disease in chickens. The virus mainly infects immature B lymphocytes in the bursa of Fabricius (BF). Chicken B cell line DT40, an avian leukosis virus-induced B cell line, supports very virulent IBDV (vvIBDV) infection in vitro and thereby serves as a good model for investigating the infection and pathogenesis of this virus. However, a transcriptome-wide understanding of the interaction between vvIBDV and B cells has not yet been achieved. This study aimed to employ time-course DNA microarrays to investigate gene expression patterns in DT40 cells after infection with vvIBDV strain LX. RESULTS: DT40 cells infected with vvIBDV exhibited alterations in the expression of many important host genes involved in signal transduction pathways, including MAPK signaling, PI3K/mTOR signaling, cell death and survival, BCR signaling, and antigen presentation. The changes in cellular mRNA levels identified by microarray analysis were confirmed for 8 selected genes using real-time reverse transcription-PCR. The upregulation of inflammatory cytokines and Toll-like receptors (TLRs) in the bursa of vvIBDV-infected chickens might involve excessive activation of the innate immune and inflammatory responses and contribute to tissue damage. CONCLUSIONS: The present study is the first to provide a comprehensive differential transcriptional profile of cultured DT40 cells in response to vvIBDV infection and further extends our understanding of the molecular mechanisms underlying vvIBDV infection and pathogenesis.
Agid:
5722803