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Development of An Enzyme-Linked Immunosorbent Assay for Determination of the Furaltadone etabolite, 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) in animal tissues

Luo, Peng Jie, Jiang, Wen Xiao, Beier, Ross C., Shen, Jian Zhong, Jiang, Hai Yang, Miao, Hong, Zhao, Yun Feng, Chen, Xia, Wu, Yong Ning
Biomedical and Environmental Sciences 2012 v.25 no.4 pp. 449
animal tissues, cross reaction, detection limit, drug residues, enzyme-linked immunosorbent assay, fish, furaltadone, inhibitory concentration 50, mass spectrometry, monitoring, monoclonal antibodies, polyclonal antibodies, protein binding, screening
A rapid, sensitive, and specific competitive direct enzyme-linked immunosorbent assay (cdELISA) for determination of protein bound 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) residues is described to monitor the illegal use of furaltadone. Polyclonal and monoclonal antibodies were produced in this study. Rabbit polyclonal antibodies were used in the optimized cdELISA method and exhibited negligible cross-reactivity with other compounds structurally related to AMOZ. The IC50 of the polyclonal antibody was 0.16 ng/mL. The method limit of detection in four different types of animal and fish tissues was less than 0.06 µg/kg. Recoveries ranged from 80% to 120% for fortified samples with the coefficient of variation values less than 15%. The cdELISA method was validated by a previously established liquid chromatography–tandem mass spectrometry method. These results indicate that the cdELISA method developed here is a convenient, practical tool for screening large numbers of animal and fish tissue samples for the detection of AMOZ residues.