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The effect of iron plaque on uptake and translocation of norfloxacin in rice seedlings grown in paddy soil

Yan, Dafang, Ma, Wei, Song, Xiaojing, Bao, Yanyu
Environmental science and pollution research international 2017 v.24 no.8 pp. 7544-7554
adsorption, antibiotics, apoplast, fluorescence microscopes, iron, iron oxides, norfloxacin, paddy soils, rhizosphere, rice, roots, seedlings, tissues
Although the role of iron plaque on rice root surface has been investigated in recent years, its effect on antibiotic uptake remains uncertain. In the study, pot experiment was conducted to investigate the effect of iron plaque on uptake and translocation of norfloxacin (adding 10 and 50 mg·kg⁻¹ treatments) in rice seedlings grown in paddy soil. Iron plaque was induced by adding different amounts of Fe(II) in soil. The results showed that the presence of norfloxacin can decrease the amount of iron plaque induced. After rice with iron plaque induced, norfloxacin was mainly accumulated in iron plaque on root surface, followed by inside root, but its translocation from root to other rice tissues is not observed. Iron plaque played the role of a barrier for norfloxacin uptake into rice roots under high norfloxacin concentration of 50 mg·kg⁻¹, however not that under low concentration of 10 mg·kg⁻¹. And the barrier function was the most strongest with adding Fe(II) of 30 mg·kg⁻¹ as combined action of iron plaque and rhizosphere effect. Fluorescence microscope analysis showed that norfloxacin mainly distributed in the outside of root cell, which showed its translocation as apoplastic pathway in rice. Comparing with non-rhizosphere, more norfloxacin was accumulated in rhizosphere soil. Maybe, strong root oxidization (high Eh values) induced more iron oxide formation in rhizosphere and on root surface, which led to norfloxacin’s mobility towards to rhizosphere through its strong adsorption of iron oxides and then promoted its uptake by rice on root surface.