Jump to Main Content
A new mechanism for efficient hydrocarbon electro-extraction from Botryococcus braunii
- Guionet, Alexis, Hosseini, Bahareh, Teissié, Justin, Akiyama, Hidenori, Hosseini, Hamid
- Biotechnology for biofuels 2017 v.10 no.1 pp. 39
- Botryococcus braunii, electric field, energy, energy costs, fluorescence microscopes, image analysis, microalgae, oils, renewable energy sources, screening, thin layer chromatography
- BACKGROUND: Recent understanding that specific algae have high hydrocarbon production potential has attracted considerable attention. Botryococcus braunii is a microalga with an extracellular hydrocarbon matrix, which makes it an appropriate green energy source. RESULTS: This study focuses on extracting oil from the microalgae matrix rather than the cells, eliminating the need for an excessive electric field to create electro-permeabilization. In such a way, technical limitations due to high extraction energy and cost can be overcome. Here, nanosecond pulsed electric fields (nsPEF) with 80 ns duration and 20–65 kV/cm electric fields were applied. To understand the extraction mechanism, the structure of the algae was accurately studied under fluorescence microscope; extraction was quantified using image analysis; quality of extraction was examined by thin-layer chromatography (TLC); and the cell/matrix separation was observed real-time under a microscope during nsPEF application. Furthermore, optimization was carried out by screening values of electric fields, pulse repetition frequencies, and energy spent. CONCLUSIONS: The results offer a novel method applicable for fast and continues hydrocarbon extraction process at low energy cost.