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Linkage map construction and QTL identification of P-deficiency tolerance in Oryza rufipogon Griff. at early seedling stage

Author:
Luo, Xiang-Dong, Liu, Jian, Dai, Liang-Fang, Zhang, Fan-Tao, Wan, Yong, Xie, Jian-Kun
Source:
Euphytica 2017 v.213 no.4 pp. 96
ISSN:
0014-2336
Subject:
Oryza rufipogon, Oryza sativa, backcrossing, chromosome mapping, chromosomes, genes, inbred lines, phenotypic variation, phosphorus, plant breeding, quantitative trait loci, rice, seedlings
Abstract:
Low phosphorus availability is a major factor limiting rice productivity. In this study, a population of backcross recombinant inbred lines (BILs) derived from an inter-specific cross (Oryza sativa L. × O. rufipogon Griff.) was used for genetic linkage map construction and quantitative trait locus (QTL) mapping. The results showed that a linkage map consisting of 153 markers was constructed. Twenty-one out of 231 BILs were tolerant of low-phosphorus according to the index to P-deficiency tolerance. Twenty-three QTLs on chromosomes 1, 2, 3, 7, 8, 9 and 11 were detected, of which eight QTLs showed high (22.93–37.32%) contribution to phenotypic variation. In addition, most of QTLs in this study (18 out of 23 QTLs) were located and overlapped on the chromosome 1, 3 and 11, which individually explained 6.07–34.70% phenotypic variation, indicating that there might be multiple main effect QTLs related to P-deficiency tolerance in O. rufipogon, and these QTLs might cluster in the same region. These results would provide helpful information for cloning and utilizing the P-deficiency tolerance-responsive genes from O. rufipogon.
Agid:
5724825