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Distinct association of an alphasatellite and a betasatellite with Tomato leaf curl New Delhi virus in field-infected cucurbit
- Anwar, Sidra
- Journal of general plant pathology 2017 v.83 no.3 pp. 185-188
- Cucurbita pepo, Tomato leaf curl New Delhi virus, leaf curling, leaves, papayas, polymerase chain reaction
- Leaf samples of Cucurbita pepo with yellow mosaic disease symptoms were collected in 2012. Rolling circle amplification and PCR amplification with begomovirus-specific primers confirmed the presence of an Old World bipartite begomovirus, an alphasatellite and a betasatellite. Molecular analysis of full-length sequences showed that Tomato leaf curl New Delhi virus (DNA-A) is associated with its cognate DNA-B, Papaya leaf curl betasatellite and a novel alphasatellite. To the best of our knowledge, this is the first report of an alphasatellite and a betasatellite associated with a bipartite begomovirus.