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High-throughput production of peroxidase and its biodegradation potential toward polymeric material

Khatoon, N., Sahar, N. I., Ndu, U., Ali, N., Jamal, A., Ahmed, S., Ali, M. I.
International journal of environmental science and technology 2017 v.14 no.4 pp. 755-766
Fourier transform infrared spectroscopy, Phanerochaete chrysosporium, alcohols, ammonium sulfate, analysis of variance, biodegradation, bioprocessing, biosynthesis, ferrous sulfate, glucose, lignin, lignin peroxidase, models, peroxidase, plastics, poly(vinyl chloride), polystyrenes, society, sucrose, urea, waste treatment, wastes
Plastics are polymeric materials, and their disposal is a great problem in today’s society. Large quantities of single-use plastics are used every minute throughout the world. Peroxidase enzymes play a significant role in the biodegradation of polymeric materials due to oxidoreductase capability. The objective is to determine which set of conditions optimize the production of peroxidase enzymes by Phanerochaete chrysosporium so as to degrade polymeric materials. The sequential order of parameters in terms of their relevant performance in the bioprocess was determined as urea > polyvinyl chloride > incubation time > polyethylene > veratryl alcohol > sucrose > ammonium sulfate > glucose > ferrous sulfate and polystyrene. Statistical analysis was performed by using analysis of variance which indicated the significance of model Plackett–Burman and components on the basis of F value and P value of 0.012678 < 0.05. The Fourier transform infrared spectroscopy of enzyme-treated polymer revealed structural changes at 1091, 1638 cm⁻¹. A new peak appeared at wave number 1029 and represented the aromatic ether and phenolic group as compared to control. Biosynthesis of lignin peroxidase at optimized conditions has the potential for biodegradation of recalcitrant polymeric waste, due to its oxidoreductase capability for chemically inert material in nature like lignin and can be used for waste treatment on a large scale.