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Physiological and proteomic analysis of rice (Oryza sativa L.) in flag leaf during flowering stage and milk stage under drought stress

Author:
Wang, Yuwen, Xu, Chao, Zhang, Beibei, Wu, Min, Chen, Guoxiang
Source:
Plant growth regulation 2017 v.82 no.2 pp. 201-218
ISSN:
0167-6903
Subject:
Oryza sativa, adenosine triphosphate, antioxidants, carbon dioxide, drought, electrophoresis, filling period, flowering, gels, hybrids, leaves, pathogenesis-related proteins, photosynthesis, proteolysis, proteomics, rice, water stress
Abstract:
Hybrid rice has the inferiority of early senescence during reproductive stage. Early senescence of leaves induced by drought causes a constraint on crop productivity. Hence, to better understand traits of drought responsive mechanism in hybrid rice flag leaves during sensitive reproductive stage, we performed physiological measurements and conducted 2-D electrophoresis to investigate proteomic profile of rice flag leaves at flowering and milk stage. 43 proteins showed significant changes in silver stained gels were identified by MS/MS at flowering stage and 54 proteins at milk stage. For flowering stage, inactive CO₂ assimilation and ATP synthesis as well abnormal floral development could be postulated from decreased proteins. Additionally, higher levels of defense-related proteins and antioxidases in drought-stressed leaves suggested active ROS scavenging system. For milk stage, both photosynthesis and CO₂ assimilation were impaired. Disrupted oxidative defense and proteolysis system indicated redox imbalance. Upregulation of NADP-MDH facilitated for CO₂ assimilation and antioxidant system. In conclusion, drought stress during reproductive stage impacted a lot on biological processes in rice leaves at protein level, especially energy metabolism and redox balance.
Agid:
5726304