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Conservation of microRNA-mediated regulatory networks in response to copper stress in grapevine

Author:
Leng, Xiangpeng, Wang, Peipei, Zhao, Pengcheng, Wang, Mengqi, Cui, Liwen, Shangguan, Lingfei, Wang, Chen
Source:
Plant growth regulation 2017 v.82 no.2 pp. 293-304
ISSN:
0167-6903
Subject:
Vitis, copper, gene expression regulation, homeostasis, messenger RNA, microRNA, proteins
Abstract:
Plants have developed a complex regulatory networks to appropriately regulate copper homeostasis in response to the frequently-changing availability of copper (Cu). However, little is known about miRNA-mediated Cu-regulatory networks in grapevine. To depict the regulatory mechanism of Cu-responsive microRNAs (miRNAs) in grapevine, three conserved Cu-responsive miRNAs (miR397, miR398 and miR408) that had the most copies of the Cu-response element (CuRE) were investigated in response to copper availability. It was found that the suppression of Cu-associated miRNAs followed by the up-regulation of their target genes encoding Cu-containing proteins. Four target genes such as vvi-LAC4 and vvi-LAC17 for miR397, vvi-PC and vvi-LAC12 for miR408 were experimentally verified by RLM-RACE. The results showed that these vvi-miRNAs negatively regulated expression of their target mRNAs through guiding corresponding target mRNA cleavage. Analysis of cis-elements showed that three miRNAs were not only in response to Cu stress but also involved in a complicated regulation network to respond to environmental stress. Our results are of good help for better understanding miRNA-guided Cu stress regulatory networks in grapevine.
Agid:
5726307