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Establishment of an Aqueous PEG 200-Based Deep Eutectic Solvent Extraction and Enrichment Method for Pumpkin (Cucurbita moschata) Seed Protein

Liu, Rui-Lin, Yu, Pei, Ge, Xian-Li, Bai, Xiu-Feng, Li, Xing-Qiang, Fu, Qiang
Food analytical methods 2017 v.10 no.6 pp. 1669-1680
Cucurbita moschata, analytical methods, chlorides, choline, coprecipitation, ethylene glycol, hydrogen bonding, polyethylene glycol, pumpkins, response surface methodology, solvents, temperature
A novel approach for ultrasound–microwave synergistic extraction (UMSE) of pumpkin seed protein was developed using aqueous poly (ethylene glycol) (PEG 200)-based deep eutectic solvent (DES) as a green extraction medium. Key factors controlling the extraction and optimal operating conditions were optimized by combining the one variable at a time and response surface methodology. Results showed that the PEG 200 as a hydrogen bond donor combined with choline chloride as a typical hydrogen bond acceptor had a highest extraction efficiency among different solvents. The optimal extraction parameters were optimized as follows: PEG 200-based DES concentration, 28% w/w; solid to liquid ratio, 28 g mL⁻¹; microwave power, 140 W; and extraction temperature, 43 °C. Under the optimal parameters, the actual extraction yield was 93.95 ± 0.23% (n = 3). The precipitation rate of pumpkin seed protein was 97.97% with a precipitation time of only 4 min by using an isoelectric point-ethanol-PEG 200 DES ternary co-precipitation method. Overall, this integrated method of PEG 200-based DES and UMSE exhibits a powerful tool for the rapid and efficient extraction of pumpkin seed protein.