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Physiological and proteomic response of Limonium bicolor to salinity

L. S. Wang, W. L. Li, X. W. Qi, L. Ma, W. L. Wu
Russian journal of plant physiology 2017 v.64 no.3 pp. 349-360
Limonium, amino acid metabolism, biosynthesis, cytoskeleton, energy, gels, gene expression regulation, halophytes, homeostasis, leaves, lipids, protein synthesis, proteins, proteomics, roots, salinity, salt stress, secondary metabolites, sodium chloride, superoxide dismutase, two-dimensional gel electrophoresis, vitamins
Medicinal halophyte Limonium bicolor (Bag.) Kuntze was treated with 200 mM NaCl, the physiological parameters including fresh weight, dry weight, salt gland number, MDA levels and root activity were investigated, comparative proteomic analyses of leaf and root were carried out using 2D-PAGE combined with MALDI-TOF/TOF-MS. The results showed that L. bicolor performed a positive effect on its growth under NaCl stress since increase in different extent of fr wt, dry wt, salt gland number, MDA level and SOD activity of leaves and roots, as well as root activity. More than 460 protein spots in leaves and 600 protein spots in roots were visualized and matched in 2D gels, the results of proteomic analyses showed that 45 proteins were identified in leaves and 49 proteins in roots to be differentially expressed. Based on their functions, these proteins were grouped into categories covering a wide range of molecular processes, including carbohydrate, energy, lipid, nucleotide and amino acid metabolism, glycan biosynthesis and metabolism, metabolism of cofactors and vitamins, biosynthesis of secondary metabolites, redox homeostasis, transcription, cytoskeleton and transport, as well as folding, sorting and degradation. The upregulating and downregulating of differentially- expressed proteins with different physiological function were also reflected in the physiological parameters. These results are helpful for further research work on salt response mechanisms and medicinal utilization of L. bicolor.