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Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages

Author:
Hou, Jinxing, An, Xiaopeng, Song, Yuxuan, Cao, Binyun, Yang, Heping, Zhang, Zhou, Shen, Wenzheng, Li, Yunpu
Source:
BMC genetics 2017 v.18 no.1 pp. 38
ISSN:
1471-2156
Subject:
adipokines, colostrum, filling period, gene expression regulation, goats, high-throughput nucleotide sequencing, lactation, leptin receptors, mammary development, mammary glands, microRNA, milk, milk secretion, mitogen-activated protein kinase, oxytocin, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, signal transduction, tissues
Abstract:
BACKGROUND: MicroRNAs (miRNAs) have a great influence on various physiological functions. A lot of high-throughput sequencing (HTS) research on miRNAs has been executed in the caprine mammary gland at different lactation periods (common milk lactation and dry period), but little is known about differentially expressed miRNAs in the caprine mammary gland of colostrum and peak lactation periods. RESULT: This study identified 131 differentially expressed miRNAs (P < 0.05 and log₂ colostrum normalized expression (NE)/peak lactation NE > 1 or log₂ colostrum NE/peak lactation NE < −1), including 57 known miRNAs and 74 potential novel miRNAs in the colostrum and peak lactation libraries. In addition, compared with differentially expressed miRNAs in the peak lactation period, 45 miRNAs in the colostrum lactation period were remarkably upregulated, whereas 86 miRNAs were markedly downregulated (P < 0.05 and log₂ colostrum NE/peak lactation NE > 1 or log₂ colostrum NE/peak lactation NE < −1). The expressions of 10 randomly selected miRNAs was analyzed through stem-loop real-time quantitative PCR (RT-qPCR). Their expression patterns were the same with Solexa sequencing results. Pathway analysis suggested that oestrogen, endocrine, adipocytokine, oxytocin and MAPK signalling pathways act on the development of mammary gland and milk secretion importantly. In addition, the miRNA-target-network showed that the bta-miR-574 could influence the development of mammary gland and lactation by leptin receptor (LEPR), which was in the adipocytokine signalling pathway. Chr5_3880_mature regulated mammary gland development and lactation through Serine/threonine-protein phosphatase (PPP1CA), which was in the oxytocin signalling pathway. CONCLUSIONS: Our finding suggested that the profiles of miRNAs were related to the physiological functions of mammary gland in the colostrum and peak lactation periods. The biological features of these miRNAs may help to clarify the molecular mechanisms of lactation and the development of caprine mammary gland.
Agid:
5728003