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Effects of starvation and feeding on blood chemistry, fatty acid composition and expression of vitellogenin and fatty acid-binding protein genes in female swimming crab Portunus trituberculatus broodstock

Author:
Ding, Liyun, Fu, Huiyun, Hou, Yingmei, Jin, Min, Sun, Peng, Zhou, Qicun
Source:
Fisheries science 2017 v.83 no.3 pp. 455-464
ISSN:
0919-9268
Subject:
Portunus trituberculatus, blood glucose, blood serum, breeding stock, cholesterol, clams, crabs, fatty acid composition, fatty acid-binding proteins, fatty acids, females, genes, gonadosomatic index, gonads, hepatopancreas, protein content, starvation, tanks, vitellogenin
Abstract:
Portunus trituberculatus broodstock were stocked in plastic tanks to evaluate the effects of starvation and feeding on gonadal development, blood chemistry, fatty acid composition, and expression of vitellogenin (Vtg) and fatty acid-binding protein genes (FABP) in females. Two treatments (starved and fed) were randomly assigned to triplicate groups of 90 swimming crab broodstock (approximately 230 ± 45 g). In the starved treatment, crabs were starved for 30 days, whereas in the fed treatment crabs were fed once a day with clams. The gonadosomatic index decreased significantly in starved crabs (P < 0.05), as did the serum glucose and cholesterol concentrations; conversely, the total protein concentration in serum significantly increased (P < 0.05). In the ovary, there was a significant relative decline of 18:0, 16:1n-7 and 20:1n-9 fatty acids and relative increases of 20:4n-6, 22:6n-3, 18:1n-9 and 20:5n-3 in starved crabs compared to fed crabs (P < 0.05). Relative expression of Vtg in the ovary decreased significantly in starved crabs (P < 0.05), while there was no significant difference in hepatopancreas Vtg expression between starved and fed crabs (P > 0.05). Starvation suppressed gonadal development in female swimming crab broodstock.
Agid:
5728096