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Pre-Clinical Study for the Antidiabetic Potential of Selenium Nanoparticles

Ahmed, Hanaa H., Abd El-Maksoud, Mohamed Diaa, Abdel Moneim, Ahmed E., Aglan, Hadeer A.
Biological trace element research 2017 v.177 no.2 pp. 267-280
adults, animal disease models, antioxidant activity, blood glucose, blood serum, catalase, females, glucagon, glucose, glutathione, glutathione peroxidase, glutathione-disulfide reductase, histopathology, immunohistochemistry, inflammation, insulin, insulin secretion, islets of Langerhans, malondialdehyde, metformin, nanoparticles, necrosis, nitric oxide, noninsulin-dependent diabetes mellitus, oxidative stress, prostaglandins, rats, selenium, sodium chloride, streptozotocin, superoxide dismutase, tumor necrosis factor-alpha
This research was delineated to explore the efficacy of selenium nanoparticles delivered in liposomes (L-Se) in the mitigation of type-2 diabetes mellitus. Adult female Wistar rats were assigned into four groups: group I, the normal control group in which the rats received normal saline solution orally; group II, the diabetic control group in which the rats were injected intraperitoneally with a single dose of streptozotocin (STZ) for induction of diabetes; group III, the metformin (Met)-treated group in which the diabetic rats were treated orally with Met; and group IV, the L-Se-treated group in which the diabetic rats were treated orally with L-Se. All treatments were delivered for 21 days. Blood and pancreas tissue samples were obtained for biochemical analysis, immunohistochemical examinations, and histopathological investigation. The L-Se-treated group showed significant drop in serum glucose and pancreatic malondialdehyde (MDA), nitric oxide (NO), tumor necrosis factor-α (TNF-α), and prostaglandin F2α (PGF2α) levels associated with significant rise in serum insulin and pancreatic glutathione, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) values, in addition to significant improvement in the immunohistochemical indices (insulin and glucagon). Aforementioned results are appreciated by the histopathological findings of pancreatic tissue. In conclusion, our data have brought about compelling evidence favoring the antidiabetic potency of elemental selenium nanoparticles delivered in liposomes through preservation of pancreatic β cell integrity with consequent increment of insulin secretion and in turn glucose depletion, repression of oxidative stress, potentiation of the antioxidant defense system, and inhibition of pancreatic inflammation.