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Antimicrobial activity of allyl isothiocyanate used to coat biodegradable composite films as affected by storage and handling conditions

Li, Weili, Liu, Linshu, Jin, Tony Z.
Journal of food protection 2012 v.75 no.12 pp. 2234
Salmonella, air, allyl isothiocyanate, antibacterial properties, atmosphere, chitosan, coatings, films (materials), food packaging, packaging materials, statistical analysis, storage temperature, storage time, sugar beet pulp, surface area, washing
We evaluated the effects of storage and handling conditions on the antimicrobial activity of biodegradable composite films (polylactic acid and sugar beet pulp) coated with allyl isothiocyanate (AIT). Polylactic acid (PLA) and chitosan were incorporated with AIT and coated on one side of the film. The films were treated at different storage conditions (storage time, storage temperature, and atmosphere) and handling conditions (washing, abrasion, and air blowing), and the antimicrobial activity of the films against Salmonella in tryptic soy broth was determined. The films (8.16 µL AIT/ cm2 surface area) significantly (P < 0.05) inhibited the growth of Salmonella during 24 h incubation at 22°C while the populations of Salmonella in controls increased from ca. 4 to over 8 log CFU/ml, indicating a minimum inactivation of 4 log CFU/ml on films in comparison to the growth on controls. Statistical analyses indicated that storage time, storage temperature, and surface abrasion affected antimicrobial activity of the films significantly (P < 0.05). However, the differences in microbial reduction between those conditions were less than 0.5 log cycle. Results suggest that the films’ antimicrobial properties are stable under practical storage and handling conditions and these antimicrobial films have potential applications in food packaging.