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Characterization of Salvia Miltiorrhiza ethanol extract as an anti-osteoporotic agent

Cui, Yan, Bhandary, Bidur, Marahatta, Anu, Lee, Geum-Hwa, Li, Bo, Kim, Do-Sung, Chae, Soo-Wan, Kim, Hyung-Ryong, Chae, Han-Jung
BMC complementary and alternative medicine 2011 v.11 no.1 pp. 120
Oriental traditional medicine, Salvia miltiorrhiza, alternative medicine, biochemistry, blood sampling, blood serum, bone density, cardiovascular diseases, dual-energy X-ray absorptiometry, ethanol, females, liver, micro-computed tomography, microscopy, nitric oxide, osteoclasts, osteoporosis, oxidative stress, rats, tibia
BACKGROUND: Salvia miltiorrhiza (SM) has long been used as a traditional oriental medicine for cardiovascular disease. Accumulating evidence also indicates that SM has anti-osteoporotic effects. This study was conducted to examine the SM-induced anti-osteoporotic effect and its possible mechanisms with various doses of SM. METHODS: We studied Sprague-Dawley female rats aged 12 weeks, divided into six groups: sham-operated control (SHAM), OVX rats supplemented with SM (1, 3, 10 and 30 mg/kg) orally for 8 weeks. At the end of the experiment, blood samples were collected and biochemistry analysis was performed. Specimens from both tibia and liver were processed for light microscopic examination. DEXA and μ-CT analyses of the tibia were also performed. RESULTS: SM treatment significantly ameliorated the decrease in BMD and trabecular bone mass according to DEXA and trabecular bone architecture analysis of trabecular bone structural parameters by μ-CT scanning. In serum biochemical analysis, SM decreased the released TRAP-5b, an osteoclast activation marker and oxidative stress parameters including MDA and NO induced by OVX. CONCLUSIONS: The preventive effect of SM was presumably due to its anti-oxidative stress partly via modulation of osteoclast maturation and number. In current study, SM appears to be a promising osteoporosis therapeutic natural product.