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Bioassay-guided isolation of glycoprotein SPG-56 from sweet potato Zhongshu-1 and its anti-colon cancer activity in vitro and in vivo

Author:
Wang, Meimei, Ma, Hang, Tian, Cheng, Liu, Shanshan, Ye, Xiaoli, Zhou, Deqi, Li, Yanlin, Hui, Nie, Li, Xuegang
Source:
Journal of functional foods 2017 v.35 pp. 315-324
ISSN:
1756-4646
Subject:
Western blotting, amino acids, antineoplastic activity, apoptosis, chemical precipitation, colorectal neoplasms, electrophoresis, flow cytometry, glycoproteins, high performance liquid chromatography, humans, immunohistochemistry, mice, molecular weight, neoplasm cells, protein content, sugars, sweet potatoes
Abstract:
This study is aimed at separating and identifying a new glycoprotein from sweet potato (SPG) and detecting its anti-colon cancer bioactivity. The SPG-56 was obtained by water extraction, isoelectric precipitation, chromatography and electrophoresis. The compositions of sugar and protein in SPG-56 were identified with high performance liquid chromatography and amino acid analyzer, and the antitumor activities were explored using BALB/c nude mice and human colon cancer cells (HCT-116). Results showed that the molecular weight of SPG-56 is 56 KD. Relative sugar and protein contents of SPG-56 are 2.9% and 97.1%, comprising more than 6 types of sugars and 15 kinds of amino acids. Animal experiments showed that orally administrated SPG-56 could obviously suppress the tumor growth. Results from flow cytometry, western blotting and immunohistochemistry indicated that SPG-56 inhibits cancer by inducing apoptosis. These results suggest that SPG-56 would potentially delay the development of colon cancer.
Agid:
5735168