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Rapid and sensitive immunoassays for the detection of lomefloxacin and related drug residues in bovine milk samples

Mukunzi, Daniel, Isanga, Joel, Suryoprabowo, Steven, Liu, Liqiang, Kuang, Hua
Food and agricultural immunology 2017 v.28 no.4 pp. 599-611
animal-based foods, cross reaction, detection limit, drug residues, enzyme-linked immunosorbent assay, immunoaffinity chromatography, inhibitory concentration 50, lomefloxacin, milk, monoclonal antibodies, norfloxacin
The misuse and illegal use of fluoroquinolones (FQs) in animal-based food products have drawn considerable attention in several countries. As a result, there has been an increased demand for efficient detection methods of FQs in food products. In this study, we developed an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic strip device based on a monoclonal antibody against lomefloxacin (LFLX), a second generation FQ. Ic-ELISA had an IC ₅₀ value of 0.19 ng/mL with a limit of detection of 0.04 ng/mL in 0.01 M phosphate-buffered saline (PBS). The intra- and inter-assay recovery rates of LFLX in bovine milk samples were 98.02–107.40% and 100.65–107.82%. The immunochromatographic assay of LFLX in PBS and spiked bovine milk samples had visual cutoff values at 1 and 5.0 ng/mL, respectively, with significant cross-reactivity with norfloxacin and enoxacin. The developed ic-ELISA and strip method may assist in the detection of FQs in foods.