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Rapid and sensitive immunoassays for the detection of lomefloxacin and related drug residues in bovine milk samples
- Mukunzi, Daniel, Isanga, Joel, Suryoprabowo, Steven, Liu, Liqiang, Kuang, Hua
- Food and agricultural immunology 2017 v.28 no.4 pp. 599-611
- animal-based foods, cross reaction, detection limit, drug residues, enzyme-linked immunosorbent assay, immunoaffinity chromatography, inhibitory concentration 50, lomefloxacin, milk, monoclonal antibodies, norfloxacin
- The misuse and illegal use of fluoroquinolones (FQs) in animal-based food products have drawn considerable attention in several countries. As a result, there has been an increased demand for efficient detection methods of FQs in food products. In this study, we developed an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic strip device based on a monoclonal antibody against lomefloxacin (LFLX), a second generation FQ. Ic-ELISA had an IC ₅₀ value of 0.19 ng/mL with a limit of detection of 0.04 ng/mL in 0.01 M phosphate-buffered saline (PBS). The intra- and inter-assay recovery rates of LFLX in bovine milk samples were 98.02–107.40% and 100.65–107.82%. The immunochromatographic assay of LFLX in PBS and spiked bovine milk samples had visual cutoff values at 1 and 5.0 ng/mL, respectively, with significant cross-reactivity with norfloxacin and enoxacin. The developed ic-ELISA and strip method may assist in the detection of FQs in foods.