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Development of indirect competitive enzyme-linked immunosorbent and immunochromatographic strip assays for carbofuran detection in fruits and vegetables
- Yao, Leijun, Liu, Liqiang, Song, Shanshan, Kuang, Hua, Xu, Chuanlai
- Food and agricultural immunology 2017 v.28 no.4 pp. 639-651
- apples, carbofuran, cell fusion, cross reaction, cucumbers, enzyme-linked immunosorbent assay, fruits, haptens, immunization, immunoaffinity chromatography, mice, monoclonal antibodies, rapid methods, toxicity
- Carbofuran is a highly toxic pesticide used in fruits and vegetables. In this study, we produced a specific and sensitive monoclonal antibody (mAb) which was prepared based on a hapten that was derivatized with benzofuranol against carbofuran. Following mice immunization and cell fusion, we obtained three monoclonal cell lines: 6D5, 3H2, and 3C6. The cell line 3H2 generated mAb with the highest affinity and sensitivity. The half maximal inhibitory concentration was 0.3 ng/mL, and the cross-reactivity was <1%. Based on this mAb, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic test strip (ICS) assay were developed to detect carbofuran residues in cucumbers and apples. The working range of ic-ELISA was 0.1–1 ng/mL, and the cutoff value of ICS was 1 ng/mL. The analytical recovery of carbofuran in cucumber and apple samples ranged from 81% to 97%. Both methods represent rapid screening tools for carbofuran detection in fruits and vegetables.