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Grape seed extract (GSE) modulates campylobacter pro-inflammatory response in human intestinal epithelial cell lines

Silvan, Jose Manuel, Mingo, Elisa, Martinez-Rodriguez, Adolfo J.
Food and agricultural immunology 2017 v.28 no.5 pp. 739-753
Campylobacter, campylobacteriosis, disease prevention, disease severity, dose response, epithelial cells, grape seed extract, human cell lines, inflammation, interleukin-6, interleukin-8, intestinal mucosa, patients, secretion
The main aim of this study was to investigate the effect of Grape seed extract (GSE) on Campylobacter induced-cytokine production by human intestinal cell lines. With this purpose, Caco-2 and HT-29 cells were challenged with culture supernatants from several strains of C. jejuni and levels of secreted IL-6, IL-8, and MCP-1 were measured. Cytokine production was higher in HT-29 than in Caco-2 cells, showing different levels of secretion depending of the epithelial cells origin. Also, infective isolates coming from campylobacteriosis patients rendered higher levels of pro-inflammatory cytokines. Co-treatment and pre-treatment of HT-29 cells with GSE and C. jejuni significantly reduced cytokines production in a dose-dependent manner. These results make this natural product a putative nutritional tool for use in the treatment of campylobacteriosis, which could contribute to improve disease prevention or reduce disease severity.