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Recycled de-Oiled Algal Biomass Extract as a Feedstock for Boosting Biodiesel Production from Chlorella minutissima
- Arora, Neha, Patel, Alok, Pruthi, Parul A, Pruthi, Vikas
- Applied biochemistry and biotechnology 2016 v.180 no.8 pp. 1534-1541
- Chlorella minutissima, biodiesel, biomass production, carbon, cost effectiveness, droplets, fatty acid composition, feedstocks, fuel production, lipid content, lipids, microalgae, models, neutralization, photosynthesis, pigments, protein content
- The investigation for the first time assesses the efficacy of recycled de-oiled algal biomass extract (DABE) as a cultivation media to boost lipid productivity in Chlorella minutissima and its comparison with Bold’s basal media (BBM) used as control. Presence of organic carbon (3.8 ± 0.8 g/l) in recycled DABE resulted in rapid growth with twofold increase in biomass productivity as compared to BBM. These cells expressed four folds higher lipid productivity (126 ± 5.54 mg/l/d) as compared to BBM. Cells cultivated in recycled DABE showed large sized lipid droplets accumulating 54.12 % of lipid content. Decrement in carbohydrate (17.76 %) and protein content (28.12 %) with loss of photosynthetic pigments compared to BBM grown cells were also recorded. The fatty acid profiles of cells cultivated in recycled DABE revealed the dominance of C16:0 (39.66 %), C18:1 (29.41 %) and C18:0 (15.82 %), respectively. This model is self-sustained and aims at neutralizing excessive feedstock consumption by exploiting recycled de-oiled algal biomass for cultivation of microalgae, making the process cost effective.