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Isolation and Characterization of Altererythrobacter sp. DT for Biotreatment of and Sulfur Production from Thiosulfate-Bearing Industrial Wastewater

Author:
Gu, XiangYang, Dou, PeiChong
Source:
Water, air, and soil pollution 2017 v.228 no.1 pp. 28
ISSN:
0049-6979
Subject:
Altererythrobacter, bacteria, bioremediation, carbon, metabolism, methionine, microbial growth, nucleotide sequences, pH, peptones, ribosomal DNA, saline soils, sequence analysis, sulfates, sulfur, temperature, thiosulfates, wastewater, yeast extract
Abstract:
The objectives of the present study are to isolate thiosulfate-degrading bacterium and optimize its degradative conditions including temperature, pH, and thiosulfate concentrations required for bioremediation purposes. A heterotrophic thiosulfate-degrading bacterial strain DT was successfully isolated from saline soil and identified as Altererythrobacter sp. based on its physicochemical properties and 16S rDNA sequence analysis. It was a naturally occurring methionine auxotrophic strain that utilized only peptone, yeast extract, or several amino acids as the sole carbon source. Altererythrobacter sp. DT degraded thiosulfate via a distinctive disproportionation reaction which was characterized by accumulation of sulfate and elemental sulfur at a molar ratio of 1:1. Optimal conditions for both bacterial growth and thiosulfate metabolism were 25–30 °C and pH 6, respectively. In a fed-batch treatment system receiving liquid polysulfide wastewater, a high degradation rate of 407.3 mg S₂O₃²⁻/(L h) and an elemental sulfur yield of nearly 50% were achieved for immobilized DT cells, indicating great potential of strain DT for future application in the treatment of and microbial production of elemental sulfur from thiosulfate-bearing industrial wastewater.
Agid:
5737981