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A deep sequencing reveals significant diversity among dominant variants and evolutionary dynamics of avian leukosis viruses in two infectious ecosystems

Author:
Meng, Fanfeng, Dong, Xuan, Hu, Tao, Chang, Shuang, Fan, Jianhua, Zhao, Peng, Cui, Zhizhong
Source:
BMC veterinary research 2016 v.12 no.1 pp. 287
ISSN:
1746-6148
Subject:
Avian leukosis virus, cell culture, chickens, ecosystems, entropy, flocks, genes, genetic variation, high-throughput nucleotide sequencing, liver neoplasms, molecular epidemiology, mutation, regulatory sequences, selection pressure, specific pathogen-free animals, transcription (genetics), viruses
Abstract:
BACKGROUND: As a typical retrovirus, the evolution of Avian leukosis virus subgroup J (ALV-J) in different infectious ecosystems is not characterized, what we know is there are a cloud of diverse variants, namely quasispecies with considerable genetic diversity. This study is to explore the selection of infectious ecosystems on dominant variants and their evolutionary dynamics of ALV-J between DF1 cells and specific-pathogen-free (SPF) chickens. High-throughput sequencing platforms provide an approach for detecting quasispecies diversity more fully. RESULTS: An average of about 20,000 valid reads were obtained from two variable regions of gp85 gene and LTR-U3 region from each sample in different infectious ecosystems. The top 10 dominant variants among ALV-J from chicken plasmas, DF1 cells and liver tumor were completely different from each other. Also there was a difference of shannon entropy and global selection pressure values (ω) in different infectious ecosystems. In the plasmas of two chickens, a large portion of quasispecies contained a 3-peptides “LSD” repeat insertion that was only less than 0.01% in DF1 cell culture supernatants. In parallel studies, the LTR-U3 region of ALV-J from the chicken plasmas demonstrated more variants with mutations in their transcription regulatory elements than those from DF1 cells. CONCLUSIONS: Our data taken together suggest that the molecular epidemiology based on isolated ALV-J in cell culture may not represent the true evolution of virus in chicken flocks in the field. The biological significance of the “LSD” insert and mutations in LTR-U3 needs to be further studied.
Agid:
5738173