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D-Xylose fermentation, xylitol production and xylanase activities by seven new species of Sugiyamaella

Author:
Sena, Letícia M. F., Morais, Camila G., Lopes, Mariana R., Santos, Renata O., Uetanabaro, Ana P. T., Morais, Paula B., Vital, Marcos J. S., de Morais, Marcos A., Jr., Lachance, Marc-André, Rosa, Carlos A.
Source:
Antonie van Leeuwenhoek 2017 v.110 no.1 pp. 53-67
ISSN:
0003-6072
Subject:
ecosystems, ethanol, fermentation, genes, internal transcribed spacers, new species, ribosomal RNA, sequence analysis, sugarcane bagasse, wood, xylanases, xylitol, xylose, yeasts
Abstract:
Sixteen yeast isolates identified as belonging to the genus Sugiyamaella were studied in relation to D-xylose fermentation, xylitol production, and xylanase activities. The yeasts were recovered from rotting wood and sugarcane bagasse samples in different Brazilian regions. Sequence analyses of the internal transcribed spacer (ITS) region and the D1/D2 domains of large subunit rRNA gene showed that these isolates belong to seven new species. The species are described here as Sugiyamaella ayubii f.a., sp. nov. (UFMG-CM-Y607ᵀ = CBS 14108ᵀ), Sugiyamaella bahiana f.a., sp. nov. (UFMG-CM-Y304ᵀ = CBS 13474ᵀ), Sugiyamaella bonitensis f.a., sp. nov. (UFMG-CM-Y608ᵀ = CBS 14270ᵀ), Sugiyamaella carassensis f.a., sp. nov. (UFMG-CM-Y606ᵀ = CBS 14107ᵀ), Sugiyamaella ligni f.a., sp. nov. (UFMG-CM-Y295ᵀ = CBS 13482ᵀ), Sugiyamaella valenteae f.a., sp. nov. (UFMG-CM-Y609ᵀ = CBS 14109ᵀ) and Sugiyamaella xylolytica f.a., sp. nov. (UFMG-CM-Y348ᵀ = CBS 13493ᵀ). Strains of the described species S. boreocaroliniensis, S. lignohabitans, S. novakii and S. xylanicola, isolated from rotting wood of Brazilian ecosystems, were also compared for traits relevant to xylose metabolism. S. valenteae sp. nov., S. xylolytica sp. nov., S. bahiana sp. nov., S. bonitensis sp. nov., S. boreocarolinensis, S. lignohabitans and S. xylanicola were able to ferment D-xylose to ethanol. Xylitol production was observed for all Sugiyamaella species studied, except for S. ayubii sp. nov. All species studied showed xylanolytic activity, with S. xylanicola, S. lignohabitans and S. valenteae sp. nov. having the highest values. Our results suggest these Sugiyamaella species have good potential for biotechnological applications.
Agid:
5739910