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Time-course of ionic responses and proteomic analysis of a Tibetan wild barley at early stage under salt stress

Author:
Shen, Qiufang, Fu, Liangbo, Qiu, Long, Xue, Feng, Zhang, Guoping, Wu, Dezhi
Source:
Plant growth regulation 2017 v.81 no.1 pp. 11-21
ISSN:
0167-6903
Subject:
Hordeum vulgare, artificial selection, barley, genetic variation, grain crops, monodehydroascorbate reductase (NADH), peroxidases, phosphoglycerate kinase, photosynthesis, potassium, proteins, proteomics, reactive oxygen species, root growth, roots, salinity, salt stress, salt tolerance, shoots, sodium, stress tolerance, triose-phosphate isomerase
Abstract:
Barley (Hordeum vulgare L.) is well known for its relatively high salt tolerance among cereal crops. However, the genetic variation of cultivated barley becomes narrower due to continuous artificial selection and breeding processes. Compared with cultivated barley, wild barley contains wider genetic variation and abundant sources for abiotic stress tolerance, considering as an elite resource for mechanism study on salt tolerance. In this study, Tibetan wild barley accession XZ113 identified with high salt tolerance, was used to investigate ionic responses and to identify proteins involved in salt tolerance in roots and shoots at early stage of salt stress, during 48 h. Exposed to salinity, shoot growth is more sensitive than root growth. Conversely, K/Na ratio in the shoots was larger than that in the roots, and both were above 1.0. Steady-state K⁺ flux experiment showed XZ113 had a strong K⁺-retaining ability under salt stress, maybe contributing to its good performance of the absolute growth rate. Proteomic results suggested that monodehydroascorbate reductase and peroxidases related to reactive oxygen species scavenging in the roots and phosphoglycerate kinase, triosephosphate isomerase and sedoheptulose-1,7-bisphosphatase associated with photosynthesis and metabolisms in the shoots, played important roles in salt tolerance at early stage of salinity in wild barley.
Agid:
5739978