U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

Luminescence screening of enrofloxacin and ciprofloxacin residues in swine liver after dispersive liquid - liquid microextraction cleanup

Author:
Guoying Chen, Qiongqiong Li
Source:
Journal of Agricultural and Food Chemistry 2013 v.61 no. pp. 98-102
ISSN:
1520-5118
Subject:
antibiotic residues, swine, sodium chloride, screening, microextraction, luminescence, liver, enrofloxacin, ciprofloxacin, solvents
Abstract:
A luminescence method was developed to screen residues of enrofloxacin (ENRO) and its metabolite, ciprofloxacin (CIPRO), in swine liver. Target analytes were extracted in acetonitrile/1.5% trifluoroacetic acid-NaCl, cleaned up by dispersive liquid-liquid microextraction (DLLME), and finally detected by terbium-sensitized luminescence (TSL) using a time-resolved luminescence photometer. CIPRO yielded a slightly lower TSL response than ENRO, so a common threshold was derived from CIPRO and applied to both Fluoroquinolones. Among 37 samples randomly spiked with CIPRO or ENRO up to 1 ug/g, all 19 samples spiked above 500 ng/g tolerance were correctly screened as positive with no false negatives, but 3 of 18 spiked below 500 ng/g were classified as positive. This method minimized use of chlorinated solvents, and significantly improved sample throughput.
Agid:
57407
Handle:
10113/57407