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Luminescence screening of enrofloxacin and ciprofloxacin residues in swine liver after dispersive liquid - liquid microextraction cleanup
- Guoying Chen, Qiongqiong Li
- Journal of Agricultural and Food Chemistry 2013 v.61 no. pp. 98-102
- antibiotic residues, swine, sodium chloride, screening, microextraction, luminescence, liver, enrofloxacin, ciprofloxacin, solvents
- A luminescence method was developed to screen residues of enrofloxacin (ENRO) and its metabolite, ciprofloxacin (CIPRO), in swine liver. Target analytes were extracted in acetonitrile/1.5% trifluoroacetic acid-NaCl, cleaned up by dispersive liquid-liquid microextraction (DLLME), and finally detected by terbium-sensitized luminescence (TSL) using a time-resolved luminescence photometer. CIPRO yielded a slightly lower TSL response than ENRO, so a common threshold was derived from CIPRO and applied to both Fluoroquinolones. Among 37 samples randomly spiked with CIPRO or ENRO up to 1 ug/g, all 19 samples spiked above 500 ng/g tolerance were correctly screened as positive with no false negatives, but 3 of 18 spiked below 500 ng/g were classified as positive. This method minimized use of chlorinated solvents, and significantly improved sample throughput.