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Impact of Electron Beam on Storage Protein Subunits, In Vitro Protein Digestibility and Trypsin Inhibitor Content in Soybean Seeds

Author:
Kumar, Vineet, Rani, Anita, Hussain, Lulua, Jha, Priyamvada, Pal, Vijay, Petwal, V. C., Dwivedi, Jishnu
Source:
Food and bioprocess technology 2017 v.10 no.2 pp. 407-412
ISSN:
1935-5130
Subject:
beta-conglycinin, densitometry, digestible protein, genotype, glycinin, polyacrylamide gel electrophoresis, protein composition, protein subunits, seeds, soybeans, storage proteins, trypsin inhibitors
Abstract:
The present investigation was carried out to study the effect of 4.8, 9.2, 15.3 and 21.2 kGy of electron beam (EB)-irradiation on major storage proteins viz. glycinin (11S) and β-conglycinin (7S), in vitro protein digestibility (IVPD), and trypsin inhibitor (TI) content in seeds of three soybean genotypes. Densitometry of SDS-PAGE protein profile revealed significant (P < 0.05) reduction in α’, α and β subunits of 7S fraction at all doses. This reduction was higher (P < 0.05) than the decline observed in acidic and basic subunit of 11S fraction. Basic subunit registered significant (P < 0.05) increase at specific doses in two genotypes. All the doses induced significant (P < 0.05) increase in IVPD, and the increase due to 9.2, 15.3 and 21.2 kGy was higher (P < 0.05) compared to 4.8 kGy dose. The impact on TI content was genotype-dependent. The study showed a higher (P < 0.05) decline in the concentration of 7S fraction compared to 11S fraction and improvement in IVPD of soybean seeds due to exposure to EB-irradiation, which may influence the functional and nutritional value of soy products processed from them.
Agid:
5740712