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Inducible gene expression system by 3-hydroxypropionic acid

Author:
Zhou, Shengfang, Ainala, Satish Kumar, Seol, Eunhee, Nguyen, Trinh Thi, Park, Sunghoon
Source:
Biotechnology for biofuels 2015 v.8 no.1 pp. 169
ISSN:
1754-6834
Subject:
Pseudomonas denitrificans, excretion, gene expression, gene expression regulation, genes, industrial applications, microorganisms, regulatory proteins, transcription (genetics), transcription factors
Abstract:
BACKGROUND: 3-Hydroxypropionic acid (3-HP) is an important platform chemical that boasts a variety of industrial applications. Gene expression systems inducible by 3-HP, if available, are of great utility for optimization of the pathways of 3-HP production and excretion. RESULTS: Here we report the presence of unique inducible gene expression systems in Pseudomonas denitrificans and other microorganisms. In P. denitrificans, transcription of three genes (hpdH, mmsA and hbdH-4) involved in 3-HP degradation was upregulated by 3-HP by the action of a transcriptional regulator protein, LysR, and a cis-acting regulatory site for LysR binding. Similar inducible systems having an LysR transcriptional regulator were identified in other microorganisms that also could degrade 3-HP. A docking study showed that the 3-HP binding pocket is located between the N-terminal helix-turn-helix motif and the C-terminal cofactor-binding domain. CONCLUSIONS: This LysR-regulated 3-HP-inducible system should prove useful for control of the level of gene expression in response to 3-HP.
Agid:
5741921