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Genetic mapping of diagnostic markers for the Lg 2 locus conferring ligules in Triticum aestivum L. and derived from Aegilops tauschii Coss.

Author:
Amagai, Y., Kuboyama, T., Watanabe, N.
Source:
Euphytica 2017 v.213 no.3 pp. 59
ISSN:
0014-2336
Subject:
Aegilops tauschii, Hordeum vulgare, Triticum aestivum, alleles, barley, chromosome mapping, hexaploidy, loci, mutants, nucleotide sequences, phenotype, plant breeding, progeny, wheat
Abstract:
It has been suggested that the non-allelic lg ₂ and Lg ᵗ alleles, that confer liguleless phenotypes, are present in chromosome 2D. We hypothesized that the naturally occurring liguleless phenotype present in polyploid wheat was a typical example of loss of dominant duplicated alleles, a process that has been described as genetic diploidisation. In the present study, we obtained the base sequence of the candidate of Lg ₂ gene through a “blastn” query with the upstream region of HvLG, the candidate gene sequence for the ligule locus in barley (Hordeum vulgare), and developed deletion markers of the Lg ₂ locus from Ae. tauschii. We applied the deletion markers to locate the Lg ₂ locus in hexaploid wheat. The F₂ progeny of ANK-33/SynW-5 segregated in a 3 liguled:1 liguleless ratio. The Lg ₂ gene was completely linked to markers G3489_2DL12del and G3489_2DL11del and segregated in a 1:2:1 ratio. Liguleless Mutant, a liguleless mutant of Aegilops tauchii, was determined by the dominant Lg ᵗ gene. In Ae. tauschii, the F₂ progeny of KU2126/Liguleless Mutant, marker Atlg_2DL19del segregated in a 1:2:1 ratio independently of the 3 liguleless:1 liguled segregation. The results indicate that three deletion markers can be used to mark the Lg ₂ locus in chromosome 2D of synthetic and hexaploid wheat.
Agid:
5743269