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Cyclophilin B facilitates the replication of Orf virus

Author:
Zhao, Kui, Li, Jida, He, Wenqi, Song, Deguang, Zhang, Ximu, Zhang, Di, Zhou, Yanlong, Gao, Feng
Source:
Virology journal 2017 v.14 no.1 pp. 114
ISSN:
1743-422X
Subject:
Orf virus, RNA interference, Western blotting, cyclophilins, cyclosporine, genes, peptidylprolyl isomerase, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, suppression subtractive hybridization, tissue culture, viruses
Abstract:
BACKGROUND: Viruses interact with host cellular factors to construct a more favourable environment for their efficient replication. Expression of cyclophilin B (CypB), a cellular peptidyl-prolyl cis-trans isomerase (PPIase), was found to be significantly up-regulated. Recently, a number of studies have shown that CypB is important in the replication of several viruses, including Japanese encephalitis virus (JEV), hepatitis C virus (HCV) and human papillomavirus type 16 (HPV 16). However, the function of cellular CypB in ORFV replication has not yet been explored. METHODS: Suppression subtractive hybridization (SSH) technique was applied to identify genes differentially expressed in the ORFV-infected MDBK cells at an early phase of infection. Cellular CypB was confirmed to be significantly up-regulated by quantitative reverse transcription-PCR (qRT-PCR) analysis and Western blotting. The role of CypB in ORFV infection was further determined using Cyclosporin A (CsA) and RNA interference (RNAi). Effect of CypB gene silencing on ORFV replication by 50% tissue culture infectious dose (TCID₅₀) assay and qRT-PCR detection. RESULTS: In the present study, CypB was found to be significantly up-regulated in the ORFV-infected MDBK cells at an early phase of infection. Cyclosporin A (CsA) exhibited suppressive effects on ORFV replication through the inhibition of CypB. Silencing of CypB gene inhibited the replication of ORFV in MDBK cells. In conclusion, these data suggest that CypB is critical for the efficient replication of the ORFV genome. CONCLUSIONS: Cellular CypB was confirmed to be significantly up-regulated in the ORFV-infected MDBK cells at an early phase of infection, which could effectively facilitate the replication of ORFV.
Agid:
5744337