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An Enzyme-Linked Immunosorbent Assay to Detect Salinomycin Residues Based on Immunomagnetic Bead Clean-up

Tian, Wenhao, Zhang, Xiaoxiao, Song, Meirong, Jiang, Haiyang, Ding, Shuangyang, Shen, Jianzhong, Li, Jiancheng
Food analytical methods 2017 v.10 no.9 pp. 3042-3051
analytical methods, antibiotics, chickens, coccidiosis, detection limit, drug residues, enzyme-linked immunosorbent assay, liver, magnetic separation, monoclonal antibodies, muscles, salinomycin
Polyether antibiotics have been widely used for the treatment and prevention of coccidiosis in chicken farming. In the present study, an efficient, simple and inexpensive competitive indirect enzyme-linked immunosorbent assay (ciELISA) method based on immunomagnetic sample clean up was developed for salinomycin. Monoclonal antibodies were immobilized on the surface of carboxylic acid magnetic beads (2.8 μm in diameter). After a simple extraction, residues in sample extracts were specifically adsorbed and the supernatant removed by magnetic separation. Analytes retained on the beads were then released by elution prior to ciELISA. The limit of detection for salinomycin in chicken muscle and liver was 22 and 18 ng mL⁻¹, respectively, and the linear quantitative range was 47–653 ng mL⁻¹. Intra-assay recoveries ranged from 86.00 to 99.32%, and inter-assay recoveries were between 85.68 and 96.34%. The inhibition efficiency and sensitivity of this method was improved compared with traditional hydrophilic-lipophilic balance column clean up ciELISA. Furthermore, the convenience and repeatability of the immunomagnetic cleanup renders the new method of high value for the analysis of drug residues in complex matrices. Graphical Abstract The test method of schematic diagram