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Preparation and characterization of a novel microorganism embedding material for simultaneous nitrification and denitrification

Zeng, Ming, Li, Ping, Wu, Nan, Li, Xiaofang, Wang, Chang
Frontiers of environmental science & engineering 2017 v.11 no.6 pp. 15
Comamonadaceae, activated sludge, cell respiration, cyclodextrins, denitrification, gels, high-throughput nucleotide sequencing, microbial communities, nitrates, nitrification, nitrites, nitrogen, polyvinyl alcohol, porosity, sodium alginate, temperature
A novel microorganism embedding material was prepared to enhance the biological nitrogen removal through simultaneous nitrification and denitrification. Polyvinyl alcohol (PVA), sodium alginate (SA) and cyclodextrin (CD) were used to compose gel bead with embedded activated sludge. The effects of temperature, CD addition and concentrations of PVA and SA on nitrogen removal were evaluated. Results show that the gel bead with CD addition at 30°C contributed to the highest nitrogen removal efficiency and nitrogen removal rate of 85.4% and 2.08 mgL·(L·h)–¹, respectively. Meanwhile, negligible NO₃ – and NO₂ – were observed, proving the occurrence of simultaneous nitrification and denitrification. The High-Throughput Sequencing confirms that the microbial community mainly contained Comamonadaceae in the proportion of 61.3%. Overall, CD increased gel bead’s porosity and resulted in the high specific endogenous respiration rate and high nitrogen removal efficiency, which is a favorable additional agent to the traditional embedding material.