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Ecogeographic variability and genetic diversity associated with seed albumins, globulins and prolamins patterns in Vicia taxa from Algeria

Author:
Bechkri, Sakina, Medoukali, Imane, Khelifi, Douadi
Source:
Botanical studies 2017 v.58 no.1 pp. 27
ISSN:
1999-3110
Subject:
Vicia, albumins, cluster analysis, genetic variation, globulins, polyacrylamide gel electrophoresis, prolamins, provenance, scientific expeditions, sodium dodecyl sulfate, storage proteins, Algeria
Abstract:
Genetic variability was studied in 78 populations of locally collected Vicia L. taxa for seed albumins, globulins and prolamins patterns by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) along with an ecogeographic characterization of sites investigated. 131, 119 and 98 bands were respectively used for albumin, globulin and prolamin cluster analysis. Dendrograms based on the Jaccard index and the UPGMA method were generated and the degree of genetic diversity between and within taxa was evaluated. Five clusters were generated from albumins, six from globulins and four from prolamins patterns. The results reflect the great diversity of storage proteins and a high correlation was obtained between the three studied fractions. Several accessions present specific bands which could be used as a discriminatory marker both on intra and interspecific levels. No clear relationships were seen between the groups according to their geographical origin. Data obtained from ecogeographic investigation can be used for future collecting missions.
Agid:
5745427