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Enhanced n-butanol production by Clostridium beijerinckii MCMB 581 in presence of selected surfactant

Singh, Kajal, Gedam, Preety S., Raut, Atulkumar N., Dhamole, Pradip B., Dhakephalkar, P. K., Ranade, Dilip R.
3 Biotech 2017 v.7 no.3 pp. 161
Clostridium beijerinckii, biocompatibility, butanol, fermentation, mixing, nonionic surfactants, solvents
Extractive butanol fermentation with non-ionic surfactant, a recently explored area, has shown promising results with several advantages but is relatively less investigated. This work reports the extractive fermentation with selected non-ionic surfactants (L62 and L62D) to enhance butanol production using a high-butanol producing strain (Clostridium beijerinckii MCMB 581). Biocompatibility studies with both the surfactants showed growth. Higher concentrations of surfactant (>5%) affected the cell count. 15.3 g L⁻¹ of butanol and 21 g L⁻¹ of total solvents were obtained with 3% (v/v) L62 which was respectively, 43% (w/w) and 55% (w/w), higher than control. It was found that surfactant addition at 9th h doubled the productivity (from 0.13 to 0.31 g L⁻¹ h⁻¹ and 0.17 to 0.39 g L⁻¹ h⁻¹, respectively for butanol and total solvent). Butanol productivity obtained was 2–3 times higher than similar studies on extractive fermentation with non-ionic surfactants. Interestingly, mixing did not improve butanol production.