Main content area

A live gI/gE-deleted pseudorabies virus (PRV) protects weaned piglets against lethal variant PRV challenge

Yin, Yue, Xu, Zhiwen, Liu, Xiaowan, Li, Ping, Yang, Fan, Zhao, Jun, Fan, Yi, Sun, Xiangang, Zhu, Ling
Virus genes 2017 v.53 no.4 pp. 565-572
Aujeszky disease, DNA, Suid herpesvirus 1, antibodies, farms, fever, growth models, immune response, livestock and meat industry, mortality, piglets, vaccines, China
Emerging pseudorabies virus (PRV) variant has led to frequent outbreaks of PRV infection among Bartha-K61-vaccinated swine population in Chinese swine farms and caused high mortality in pigs of all age since late 2011. Here, we generated a gE/gI-deleted PRV (rPRVXJ-delgI/gE-EGFP) based on PRV variant strain (PRV-XJ) through homologous DNA recombination. Compared to parental strain, rPRVXJ-delgI/gE-EGFP showed similar growth kinetics in vitro. Its safety and immunogenicity were evaluated in weaned piglets. Our results showed that piglets immunized with rPRVXJ-delgI/gE-EGFP did not exhibit any clinical symptoms, and a high level of gB-specific antibody was detected. After lethal challenge with variant PRV (PRV-FJ strain), all vaccinated piglets survived without showing any clinical symptoms except slight fever within 7 days post-challenge. In unvaccinated piglets, typical clinical symptoms of pseudorabies were observed, and the piglets were all died at 5 days post-challenge. These results indicated that a live rPRVXJ-delgI/gE-EGFP vaccine could be a maker vaccine candidate to control the currently epidemic pseudorabies in China.