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A live gI/gE-deleted pseudorabies virus (PRV) protects weaned piglets against lethal variant PRV challenge
- Yin, Yue, Xu, Zhiwen, Liu, Xiaowan, Li, Ping, Yang, Fan, Zhao, Jun, Fan, Yi, Sun, Xiangang, Zhu, Ling
- Virus genes 2017 v.53 no.4 pp. 565-572
- Aujeszky disease, DNA, Suid herpesvirus 1, antibodies, farms, fever, growth models, immune response, livestock and meat industry, mortality, piglets, vaccines, China
- Emerging pseudorabies virus (PRV) variant has led to frequent outbreaks of PRV infection among Bartha-K61-vaccinated swine population in Chinese swine farms and caused high mortality in pigs of all age since late 2011. Here, we generated a gE/gI-deleted PRV (rPRVXJ-delgI/gE-EGFP) based on PRV variant strain (PRV-XJ) through homologous DNA recombination. Compared to parental strain, rPRVXJ-delgI/gE-EGFP showed similar growth kinetics in vitro. Its safety and immunogenicity were evaluated in weaned piglets. Our results showed that piglets immunized with rPRVXJ-delgI/gE-EGFP did not exhibit any clinical symptoms, and a high level of gB-specific antibody was detected. After lethal challenge with variant PRV (PRV-FJ strain), all vaccinated piglets survived without showing any clinical symptoms except slight fever within 7 days post-challenge. In unvaccinated piglets, typical clinical symptoms of pseudorabies were observed, and the piglets were all died at 5 days post-challenge. These results indicated that a live rPRVXJ-delgI/gE-EGFP vaccine could be a maker vaccine candidate to control the currently epidemic pseudorabies in China.