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Arenimonas alkanexedens sp. nov., isolated from a frozen soil sample

Zhu, Jie, Wang, Hui-Min, Zhang, Qi, Dong, Wei-Wei, Kong, De-Long, Wang, Yan-Wei, Song, Jin-Long, Jiang, Xu, Zhao, Shu-Miao, Zhang, Wei, Ruan, Zhi-Yong
Antonie van Leeuwenhoek 2017 v.110 no.8 pp. 1027-1034
DNA, anaerobes, bacteria, chemotaxonomy, fatty acids, frozen soils, isoprenoids, nucleic acid hybridization, nucleotide sequences, pH, phenotype, phosphatidylethanolamines, phylogeny, ribosomal RNA, sequence homology, sodium chloride, ubiquinones, China
A novel facultatively anaerobic bacterium, designated strain LAM-WHM-D11ᵀ, was isolated from a frozen soil sample of China. Cells of strain LAM-WHM-D11ᵀ were observed to be Gram-stain negative, non-motile and rod-shaped. Colonies were yellowish, and circular with convex shape. Strain LAM-WHM-D11ᵀ was found to be able to grow at 4–40 °C (optimum 15 °C), pH 7.5–2.0 (optimum 9.5) and 0–2.5% NaCl (w/v) (optimum 1.5%). The 16S rRNA gene sequence similarity analysis showed that strain LAM-WHM-D11ᵀ is closely related to Arenimonas metalli CF5-1ᵀ (98.0%), Arenimonas aquaticum NA-09ᵀ (97.9%), Arenimonas donghaensis HO3-R19ᵀ (95.6%) and Arenimonas aestuarii S2-21ᵀ (95.3%). The DNA–DNA hybridization values between the isolate and A. metalli CGMCC 1.10787ᵀ, A. aquaticum KACC 14663ᵀ, A. donghaensis KACC 11381ᵀ were 41.0 ± 1.7, 44.7 ± 1.4 and 42.8 ± 1.2%, respectively. The genomic DNA G+C content was found to be 66.5 mol% as determined by the T ₘ method. The major cellular fatty acids were identified as iso-C₁₅:₀ and iso-C₁₆:₀. The major isoprenoid quinone was identified as ubiquinone 8 (Q-8). The major polar lipids were found to be diphosphatidyglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, two phospholipids and five unidentified lipids. Based on the phylogenetic, phenotypic and chemotaxonomic characteristics, strain LAM-WHM-D11ᵀ is concluded to represent a novel species within the genus Arenimonas, for which the name Arenimonas alkanexedens sp. nov. is proposed. The type strain is LAM-WHM-D11ᵀ (ACCC 19750ᵀ = JCM 30464ᵀ).