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Ionomic Screening of Field-Grown Soybean Identifies Mutants with Altered Seed Elemental Composition
- Baxter, Ivan
- The The Plant Genome 2012 v.6 no.2 pp. 1
- mineral content, mutants, nutrient content, screening, seeds, soybeans
- Soybean seeds contain high levels of mineral nutrients essential for human and animal nutrition. High throughput elemental profiling (ionomics) has identified mutants in model plant species grown in controlled environments. Here, we describe a method for identifying potential soybean ionomics mutants grown in a field setting and apply it to 975 NMU mutagenized lines. After performing a spatial correction, we identified mutants using either visual scoring of z-score plots or computational ranking of putative mutants followed by visual confirmation. Although there was a large degree of overlap between the methods, each method identified unique lines. The visual scoring approach identified 22/427 (5%) potential mutants, 70% (16/22) of which were confirmed when seeds from the same parent plant were re-grown in the field. We also performed simulations to determine an optimal strategy for screening large populations. All data from the screen is available at www.ionomicshub.org.