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A single L288I substitution in the fusion protein of bovine parainfluenza virus type 3 enhances virus growth in semi-suitable cell lines
- Matsuura, Ryosuke, Takada, Marina, Kokuho, Takehiro, Tsuboi, Takamitsu, Kameyama, Ken-ichiro, Takeuchi, Kaoru
- Archives of virology 2017 v.162 no.8 pp. 2409-2413
- Bovine parainfluenza virus 3, chickens, fibroblasts, microbial growth, vaccines
- The bovine parainfluenza virus type 3 BN-CE vaccine strain was obtained by serial passage of the BN-1 strain in chicken embryonic fibroblasts (CEF). We previously identified a substitution (L288I) in the fusion (F) protein between the two strains. To examine the effect of the substitution on CEF adaptation and attenuation, we generated a recombinant BN-1 strain with the L288I substitution in the F protein (Fᴸ²⁸⁸ᴵ-EGFP). Fᴸ²⁸⁸ᴵ-EGFP replicated more efficiently than a recombinant BN-1 strain (wt-EGFP) in semi-suitable cell lines, suggesting that the L288I substitution was established in the BN-1 strain during the process of adaptation in CEF.