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Thermoluminescence analysis and DNA comet assay to identify grapes irradiated as a quarantine treatment

Jo, Yunhee, Sanyal, Bhaskar, Ameer, Kashif, Kwon, Joong-Ho
European food research & technology 2017 v.243 no.8 pp. 1397-1403
DNA, comet assay, dose response, grapes, irradiation, minerals, quarantine, seeds, sensory evaluation, storage time, thermoluminescence
The development of reliable methods to distinguish between irradiated and non-irradiated food is essential to address the growing industrial interest in irradiation technology. DNA comet and thermoluminescence (TL) analyses were carried out to investigate radiation-induced markers in gamma-irradiated grapes (0–2 kGy). A DNA comet assay of the seeds and flesh of irradiated samples displayed tailed cells with a dose-dependent increase in tail length. In TL measurements, irradiated samples were characterized by a prominent TL peak at 180 °C with enhanced intensity. The TL ratio (TL₁/TL₂) enhanced the reliability of the results by confirming successful isolation of minerals. Irradiation was detectable for up to 6 weeks of storage, with overall acceptance qualities confirmed by a sensory evaluation. The results of the study suggest that both detection techniques could be used to identify irradiated grapes and that simultaneous use could provide a clear determination of irradiation status.