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Identification of endophytic bacterial strain RSE1 from seeds of super hybrid rice Shenliangyou 5814 (Oryza sativa L.,) and evaluation of its antagonistic activity

Author:
Liu, Yang, Bai, Feirong, Li, Ni, Wang, Weiping, Cheng, Chi
Source:
Plant growth regulation 2017 v.82 no.3 pp. 403-408
ISSN:
0167-6903
Subject:
Oryza sativa, Paenibacillus polymyxa, Ustilaginoidea, bacteria, beta-glucanase, biological control, endophytes, false smut, genes, hybrids, pathogens, plant growth, rice, seeds, taxonomy, virulent strains
Abstract:
Endophytes are related with health and growth of plants. In this study, the endophytic bacterial strain RSE1 was isolated from seeds of super hybrid rice Shenliangyou 5814 (Oryza sativa L.,). Strain RSE1 was identified as Paenibacillus polymyxa by polyphasic taxonomy identification. Through the antagonistic test with the pathogenic strain of rice false smut, Ustilaginoidea oryzae CICC 2710, it showed that the strain RSE1 had an effective antagonistic activity against this pathogen. The draft genome of strain RSE1 was sequenced by Illumina HiSeq 2000, and 3 CDSs for glucanase gene were annotated and correlated to antagonistic activity. Using specific primers to amplify the biocontrol gene in glucanase family, β-1,3-1,4-glucanase gene (gluB) was found. This study laid a scientific foundation for developing and utilizing biological bio-control bacteria agent preventing the suffering from rice false smut.
Agid:
5750825