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Isolation of Viable but Non-culturable Bacteria from Printing and Dyeing Wastewater Bioreactor Based on Resuscitation Promoting Factor

Jin, Yi, Gan, Guojuan, Yu, Xiaoyun, Wu, Dongdong, Zhang, Li, Yang, Na, Hu, Jiadan, Liu, Zhiheng, Zhang, Lixin, Hong, Huachang, Yan, Xiaoqing, Liang, Yan, Ding, Linxian, Pan, Yonglong
Current microbiology 2017 v.74 no.7 pp. 787-797
Microbacterium, Micrococcus luteus, Ochrobactrum anthropi, bacteria, bacterial communities, bioreactors, culture media, denaturing gradient gel electrophoresis, enrichment culture, fastidious bacteria, genes, most probable number technique, polymerase chain reaction, ribosomal RNA, wastewater
Printing and dyeing wastewater with high content of organic matters, high colority, and poor biochemical performance is hard to be degraded. In this study, we isolated viable but non-culturable (VBNC) bacteria from printing and dyeing wastewater with the culture media contained resuscitation promoting factor (Rpf) protein secreted by Micrococcus luteus, counted the culturable cells number with the most probable number, sequenced 16S rRNA genes, and performed polymerase chain reaction-denaturing gradient gel electrophoresis. It is obviously that the addition of Rpf in the enrichment culture could promote growth and resuscitation of bacteria in VBNC state to obtain more fastidious bacteria significantly. The identified bacteria were assigned to nine genera in the treatment group, while the two strains of Ochrobactrum anthropi and Microbacterium sp. could not be isolated from the control group. The function of isolated strains was explored and these strains could degrade the dye of Congo red. This study provides a new sight into the further study including the present state, composition, formation mechanism, and recovery mechanism about VBNC bacteria in printing and dyeing wastewater, which would promote to understand bacterial community in printing and dyeing wastewater, and to obtain VBNC bacteria from ecological environment.