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Wirkungsbezogene Analytik – Konzepte für die Fort- und Weiterentwicklung

Author:
Braeuning, Albert, Lampen, Alfonso
Source:
Journal für Verbraucherschutz und Lebensmittelsicherheit 2017 v.12 no.2 pp. 177-184
ISSN:
1661-5751
Subject:
bioassays, luciferase, mechanism of action, toxicity, toxicology
Abstract:
The concept of effect-based analytics is based on the detection of substance-induced effects in biological test systems, not on the identification of individual compounds by classic analytic chemistry. Effect-based analytics thus also allows for the detection of unknown harmful substances. Detailed knowledge about the molecular basis of toxic effects is necessary for the development of appropriate effect-based biological tests. The best-known example for such a test system is the CALUX assay for the detection of dioxin-like effects at the aryl hydrocarbon receptor by the use of a luciferase reporter system. Such test systems allow for the reliable detection of individual modes of action in toxicology. In this paper, possible further developments in effect-based analytics will be discussed, by which parallel detection of different molecular mechanisms of toxicity might be possible in the future. Furthermore, a vision of future effect-based analytics in the area of food safety is drafted.
Agid:
5752147