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LTB-Syn: a recombinant immunogen for the development of plant-made vaccines against synucleinopathies

Author:
Arevalo-Villalobos, Jaime I., Govea-Alonso, Dania O., Monreal-Escalante, Elizabeth, Zarazúa, Sergio, Rosales-Mendoza, Sergio
Source:
Planta 2017 v.245 no.6 pp. 1231-1239
ISSN:
0032-0935
Subject:
Parkinson disease, amino acids, disease course, enterotoxigenic Escherichia coli, enterotoxins, epitopes, etiology, humoral immunity, mice, oral administration, oral vaccination, recombinant fusion proteins, vaccines
Abstract:
MAIN CONCLUSION : A recombinant antigen targeting α-synuclein was produced in the plant cell rendering an immunogenic protein capable to induce humoral responses in mice upon oral administration. Synucleinopathies are neurodegenerative diseases characterized by the abnormal accumulation of α-synuclein (α-Syn, a 140 amino acid protein that normally plays various neurophysiologic roles) aggregates. Parkinson’s disease (PD) is the synucleinopathy with the highest epidemiologic impact and although its etiology remains unknown, α-Syn aggregation during disease progression pointed out α-Syn as target in the development of immunotherapies. Herein a chimeric protein, comprising the B subunit of the enterotoxin from enterotoxigenic Escherichia coli and α-Syn epitopes, was expressed in the plant cell having the potential to induce humoral responses following oral immunization. This approach will serve as the basis for the development of oral plant-based vaccines against PD with several potential advantages such as low cost, easy scale-up during production, and easy administration.
Agid:
5753829