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Arcobacter spp. in fecal samples from Brazilian farmed caimans (Caiman yacare, Daudin 1802)

Author:
Oliveira, Maria Gabriela Xavier, Pressinotti, Leandro Nogueira, Carvalho, Giovane Spínola, Oliveira, Mirela Caroline Vilela, Moreno, Luisa Zanolli, Matajira, Carlos Emilio Cabrera, Bergamo, Alessandro Spínola, Aleixo, Victor Manuel, Veiga, Alexandre Caixeta, de Souza Corsino, Elvis, Christ, Ana Paula Guarnieri, Sato, Maria Inês Zanolli, Moreno, Andrea Micke, Knöbl, Terezinha
Source:
Tropical animal health and production 2017 v.49 no.4 pp. 777-782
ISSN:
0049-4747
Subject:
Arcobacter butzleri, Arcobacter cryaerophilus, Caiman yacare, farms, feces, foodborne illness, genes, genetic variation, hemagglutinins, hemolysins, humans, polymerase chain reaction, tanks, virulence, Brazil
Abstract:
The aim of this study was to perform the identification and molecular characterization of Arcobacter cryaerophilus and Arcobacter butzleri isolated from caiman (Caiman yacare), kept at a production farm, in Brazil. Forty fecal samples were analyzed. After isolation and identification, 21/40 strains of A. butzleri and 19/40 strains of A. cryaerophilus were subjected to PCR for potential virulence gene detection. The results of the PCR showed 38/40 strains positive for the cadF, cj1349, ciaB, and tlyA genes, 39/40 strains positive for the pldA gene, and 40/40 strains positive for the mviN gene. None of the strains presented the irgA gene. Hemagglutinin (hecA gene) and hemolysin (hecB) genes were detected in 21/40 and 16/40 strains, respectively. The SE-AFLP showed a great genetic diversity, but some clonally groups were disseminated in various tanks. These data reveal that the strains presented the same virulence traits described from Arcobacter isolated from food-borne disease in humans.
Agid:
5754616