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Development of an Effective Treatment for a 5-Log Reduction of Escherichia coli in Refrigerated Pickle Products

Author:
Lu, Huiying J., Breidt Jr., Frederick, Perez-Diaz, Ilenys
Source:
Journal of food science 2013 v.78 no.2 pp. M264
ISSN:
0022-1147
Subject:
Escherichia coli O157, Salmonella, acetic acid, acid tolerance, acid treatment, acidity, bacterial contamination, benzoic acid, brining, cucumbers, decontamination, flavor, food contamination, fumaric acid, lactic acid bacteria, pH, pathogen survival, pickles, refrigeration, sodium chloride, spoilage, temperature, texture
Abstract:
Refrigerated cucumber pickle products cannot be heat processed due to the loss of characteristic sensory attributes. Typically brined refrigerated pickles contain less than 100 mM acetic acid with pH values of 3.7 to 4.0. Refrigeration (4 to 10 ◦C) helps to inhibit the growth of spoilage bacteria and maintain flavor, texture, and appearance of the pickles. Previous research has shown that pathogenic Escherichia coli strains are unusually acid resistant and survive better in refrigerated acid solutions than at higher temperatures. We found that E. coli O157:H7 can survive for 1 mo or longer at 4 ◦C in brines typical of commercial refrigerated pickles. Our objective was to develop methods to assure a 5-log reduction of pathogenic E. coli in these types of products, while maintaining the sensory characteristics. A novel brine formulation was developed, based on current commercial refrigerated pickle brines, which contained 25 mM fumaric acid, 5 mM benzoic acid, 70 mM acetic acid, and 342 mM (2%) sodium chloride, with a pH of 3.8. Sensory data indicate that this formulation did not affect flavor or other sensory attributes of the product, compared to traditional formulations. We achieved a 5-log reduction of E. coli O157:H7 at 30 ◦C for 1.52 ± 0.15 d, at 20 ◦C for 3.12 ± 0.34 d, or at 10 ◦C for 8.83 ± 0.56 d. Growth of lactic acid bacteria was also inhibited. These results can be used by manufacturers to assure a 5-log reduction in cell numbers of E. coli O157:H7 and Salmonella without a heat process during the manufacture of refrigerated pickle products.
Agid:
57549
Handle:
10113/57549