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Effect of GH and DGAT1 gene polymorphism on feeding qualities of bull calves
- Sedykh, T. A., Gladyr, E. A., Kharzinova, V. R., Gizatullin, R. S., Kalashnikova, L. A.
- Russian agricultural sciences 2017 v.43 no.1 pp. 48-52
- DNA, Hereford, alleles, bulls, calves, gene frequency, heterozygosity, liveweight gain, polymerase chain reaction, restriction fragment length polymorphism, single nucleotide polymorphism, steppes, weight gain
- The aim of this paper is to study effects of GH and DGAT1 gene polymorphisms on feeding qualities of Hereford and Limousin bull calves bred in conditions of the Cis-Ural steppe zone. SNPs of genes GH and DGAT1 are investigated by polymerase chain reaction (PCR) and DNA restriction fragment length polymorphism (RFLP). The studied population of animals was assessed by defining the allele frequency and animal genotype occurrence for the studied gene SNPs, indicators of the actual and expected heterozygosity, and Pearson’s test. A study of polymorphism C214G of gene GH revealed that genotype LL prevails in Hereford and Limousin animals, 47.37 and 57.7%, respectively, while frequency of allele L is higher in Limousin bull calves (0.731). A study of polymorphism K232A of gene DGAT1 gene in both the populations showed absence of genotypes AA, which can be related to the low number of the studied animals. Expected heterozygosity indicators of gene GH are higher than the observed ones, and the observed heterozygosity is higher for DGAT1. The number of efficient alleles for the studied genes is higher for Hereford bull calves. In general, according to Pearson’s test, both the studied populations are in equilibrium. There is a significant effect of GH gene polymorphism on live weight gain rates at the end of sagination and total and average daily weight gains during animal raising.