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Molecular and pathological studies on natural cases of bovine theileriosis

Gupta, Anamika, Gupta, Kuldip, Leishangthem, Geeta Devi, Bal, Mandeep Singh, Sood, N. K., Singh, Amarjit
Journal of parasitic diseases 2017 v.41 no.1 pp. 211-218
DNA primers, Theileria annulata, animal organs, blood, cattle, disease diagnosis, immune response, interleukin-10, interleukin-4, lymph nodes, polymerase chain reaction, sequence analysis, theileriosis, tumor necrosis factor-alpha, India
The present communication is a part of study conducted on 32 cases of bovine lymphadenopathies. Out of which, six cases of bovine theileriosis were diagnosed on the basis of peripheral blood smear examination and gross lesions in lymph nodes and visceral organs. Nested PCR using two primer sets N516/N517 and 14136/294 was conducted on samples collected from affected lymph nodes confirms Theileria annulata infection in five out of six cases. Sequencing analysis of amplified product showed 92, 94 and 93 % homology of isolate TH1_Bovine, TH2_Bovine and THEN_Bovine for T. annulata with T. annulata Tamilnadu and Pant Nagar. An upregulation of Th2 cytokines in the cases of theileriosis was observed as the level of TNF-α in individual animals varies from higher value (1028 pg/100 μg protein) to as low as 500 pg/100 μg protein. An increase in level of Th2 cytokines IL-4 and IL-10 was also observed. The present study concluded that pathological studies and cytokine analysis of lymph nodes are of paramount importance in disease diagnosis and associated immune response of the animal with lymphadenopathies.