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Electrophoretic Analysis of Natural Antioxidants in Plant and Beverage Samples Using Dynamically Coated Capillaries with Chitosan and Multiwall Carbon Nanotubes

Xu, Jing-Jing, Ye, Li-Hong, Yi, Ling, An, Mingrui, Cao, Jun, Peng, Li-Qing
Food analytical methods 2017 v.10 no.4 pp. 980-991
Chrysanthemum, antioxidants, beverages, capillary electrophoresis, carbon nanotubes, chitosan, detection limit, pH, phenolic compounds, sodium borate, sodium dodecyl sulfate
Statically, chitosan-coated capillaries were simply dynamically coated with multiwall carbon nanotubes (MWNTs) coaggregated with sodium dodecyl sulfate (SDS). The capillary electrophoresis method was used to separate natural antioxidants in plant and beverage samples. In this way, a great improvement in the electrophoretic resolution was achieved when the running buffer was composed of 50 mM SDS and 10 mM sodium borate with 6.0 μg/mL of SDS-coated MWNTs, at pH 8.0, with an applied voltage of 30 kV. Under the optimal conditions, the limits of detection and quantitation for the target analytes were in the range of 0.14–0.82 μg/mL and of 0.47–2.75 μg/mL, respectively. The mean recovery values of 83.9–106.2 % were obtained for the spiked chrysanthemum samples. This proposed method was successfully applied for determining 12 phenolic compounds in complex chrysanthemum samples.